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Promega pooled male human genomic dna
Pooled Male Human Genomic Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+genomic+dna+male/human+genomic+dna/pmc11146591__Supplemental_material-36-14-17
Average 90 stars, based on 1 article reviews
pooled male human genomic dna - by Bioz Stars, 2026-09
90/100 stars

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Article Title: ATF4 loss of heterozygosity is associated with poor overall survival in medullary thyroid carcinoma
Article Snippet: Then, the method compares the delta CT values of the test samples to a calibrator sample that contains a known number of copies of the target sequence (human genomic DNA, male and female (Catalog #G1521, G1571, Promega).

Article Title: Helicase Dependent Isothermal Amplification of DNA and RNA using Self-Avoiding Molecular Recognition Systems
Article Snippet: [ 21 ] Human genomic DNA male was from Promega (G1471, WI, USA).

Article Title: Leukemic non-nodal mantle cell lymphomas have a distinct phenotype and are associated with deletion of PARP1 and 13q14.
Article Snippet: Leukemic non-nodal mantle cell lymphoma (lMCL) is a particular subtype of mantle cell lymphoma (MCL), characterized by leukemic non-nodal disease and slow progression.. Recognition of this entity is relevant to avoid overtreatment.. Despite indolent clinical behaviour, lMCL might transform to a more aggressive disease.

Article Title: A novel method for sex determination by detecting the number of X chromosomes.
Article Snippet: A novel method for sex determination, based on the detection of the number of X chromosomes, was established.. Current methods, based on the detection of the Y chromosome, can directly identify an unknown sample as male, but female gender is determined indirectly, by not detecting the Y chromosome.. Thus, a direct determination of female gender is important because the quality (e.g., fragmentation and amelogenin-Y null allele) of the Y chromosome DNA may lead to a false result.

Article Title: Reference Size Matching, Whole-Genome Amplification, and Fluorescent Labeling as a Method for Chromosomal Microarray Analysis of Clinically Actionable Copy Number Alterations in Formalin-Fixed, Paraffin-Embedded Tumor Tissue.
Article Snippet: A minimum of 75 to 150 ng of tumor (test) DNA and 75 to 150 ng of sex-matched (Human Genomic DNA Female and Human Genomic DNA Male) reference DNA, (Promega, Madison, WI) was required for each assay.

Article Title: Second-generation sequencing of forensic STRs using the Ion Torrent™ HID STR 10-plex and the Ion PGM™.
Article Snippet: Accepted Manuscript Title: Second-generation sequencing of forensic STRs using the Ion TorrentTM HID STR 10-plex and the Ion PGMTM Author: Sarah L. Fordyce Helle Smidt Mogensen Claus Børsting Robert E. Lagacé Chien-Wei Chang Narasimhan Rajagopalan Niels Morling PII: S1872-4973(14)00202-6 DOI: http://dx.doi.org/doi:10.1016/j.fsigen.2014.09.020 Reference: FSIGEN 1236 To appear in: Forensic Science International: Genetics Received date: 16-6-2014 Revised date: 20-8-2014 Accepted date: 23-9-2014 Please cite this article as: S.L.. Fordyce, H.S.. Mogensen, C. Borsting, R.E.

Article Title: Analysis of copy number variation in men with non-obstructive azoospermia.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense WYRWOLL ET AL. 1595 compared to a pooled male control DNA (Human Genomic DNA Male, Promega, Walldorf, Germany) and hybridised against DNA oligonucleotides representing thewhole human genome.

Article Title: RAC1 Alterations Induce Acquired Dabrafenib Resistance in Association with Anaplastic Transformation in a Papillary Thyroid Cancer Patient
Article Snippet: Then, the method compares the delta CT values of the test samples to a calibrator sample that contains a known number of copies of the target sequence (human genomic DNA, male and female (Catalog # G1521, G1571, Promega, Madison, WI, USA).



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Promega male human genomic dna input
Schematic illustration of the complete sample collection to the NGS workflow. ( A ) Manual extraction of genomic <t>DNA</t> <t>(gDNA)</t> from blood. NA is purified from human peripheral blood by performing multiple mixing and magnetic bead-based purification steps. Optionally, isolated samples are quantified to normalize the output. ( B ) Purified genomic DNA undergoes fragmentation, end modification (repair and adenylation), adapter ligation, post-ligation purification steps, amplification via PCR, post-amplification purification, and library normalization to prepare it for optimal NGS analysis
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Schematic illustration of the complete sample collection to the NGS workflow. ( A ) Manual extraction of genomic <t>DNA</t> <t>(gDNA)</t> from blood. NA is purified from human peripheral blood by performing multiple mixing and magnetic bead-based purification steps. Optionally, isolated samples are quantified to normalize the output. ( B ) Purified genomic DNA undergoes fragmentation, end modification (repair and adenylation), adapter ligation, post-ligation purification steps, amplification via PCR, post-amplification purification, and library normalization to prepare it for optimal NGS analysis
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Schematic illustration of the complete sample collection to the NGS workflow. ( A ) Manual extraction of genomic DNA (gDNA) from blood. NA is purified from human peripheral blood by performing multiple mixing and magnetic bead-based purification steps. Optionally, isolated samples are quantified to normalize the output. ( B ) Purified genomic DNA undergoes fragmentation, end modification (repair and adenylation), adapter ligation, post-ligation purification steps, amplification via PCR, post-amplification purification, and library normalization to prepare it for optimal NGS analysis

Journal: BMC Genomics

Article Title: Peripheral blood to next-generation sequencing ready DNA library: a novel engineering design for automation

doi: 10.1186/s12864-024-10892-0

Figure Lengend Snippet: Schematic illustration of the complete sample collection to the NGS workflow. ( A ) Manual extraction of genomic DNA (gDNA) from blood. NA is purified from human peripheral blood by performing multiple mixing and magnetic bead-based purification steps. Optionally, isolated samples are quantified to normalize the output. ( B ) Purified genomic DNA undergoes fragmentation, end modification (repair and adenylation), adapter ligation, post-ligation purification steps, amplification via PCR, post-amplification purification, and library normalization to prepare it for optimal NGS analysis

Article Snippet: To determine the capacity of the pretreatment solution, 40 ng of male human genomic DNA input (Promega, Madison, WI, USA) was used.

Techniques: Extraction, Purification, Isolation, Modification, Adapter Ligation, Ligation, Amplification

Schematics of the consumables and the plate layout. ( A ) Overview of single-use consumable flexible tubing with a Y-junction connecting two cannulas. ( B ) Plate layout of the reagents on a consumable 384-well plate. ( C ) NAP process with fragmentation, including the cleanup procedure with specialized order of operations in Lane B/Cannula 2 (right blue box). ( D ) Library preparation process after the fragmentation of extracted gDNA with a separate order of operations in Lane A/Cannula 1 (right blue box)

Journal: BMC Genomics

Article Title: Peripheral blood to next-generation sequencing ready DNA library: a novel engineering design for automation

doi: 10.1186/s12864-024-10892-0

Figure Lengend Snippet: Schematics of the consumables and the plate layout. ( A ) Overview of single-use consumable flexible tubing with a Y-junction connecting two cannulas. ( B ) Plate layout of the reagents on a consumable 384-well plate. ( C ) NAP process with fragmentation, including the cleanup procedure with specialized order of operations in Lane B/Cannula 2 (right blue box). ( D ) Library preparation process after the fragmentation of extracted gDNA with a separate order of operations in Lane A/Cannula 1 (right blue box)

Article Snippet: To determine the capacity of the pretreatment solution, 40 ng of male human genomic DNA input (Promega, Madison, WI, USA) was used.

Techniques:

Schematic illustration of pretreatment adsorption with flexible tubing. ( A ) No pretreatment conditions where DNA of interest (gDNA/fragmented DNA/adapters) and enzyme of interest (Fragmentase/end-repair and adenylation tailing enzyme mix/ligase) are adsorbed to the surface. ( B ) Pretreatment condition where native BSA and short DNA oligos adsorb the inner tubing affinity pores. ( C ) Heated pretreatment conditions where the pretreatment is aspirated into the heating zone to denature the native BSA to increase surface area for more efficient adsorption of the sites

Journal: BMC Genomics

Article Title: Peripheral blood to next-generation sequencing ready DNA library: a novel engineering design for automation

doi: 10.1186/s12864-024-10892-0

Figure Lengend Snippet: Schematic illustration of pretreatment adsorption with flexible tubing. ( A ) No pretreatment conditions where DNA of interest (gDNA/fragmented DNA/adapters) and enzyme of interest (Fragmentase/end-repair and adenylation tailing enzyme mix/ligase) are adsorbed to the surface. ( B ) Pretreatment condition where native BSA and short DNA oligos adsorb the inner tubing affinity pores. ( C ) Heated pretreatment conditions where the pretreatment is aspirated into the heating zone to denature the native BSA to increase surface area for more efficient adsorption of the sites

Article Snippet: To determine the capacity of the pretreatment solution, 40 ng of male human genomic DNA input (Promega, Madison, WI, USA) was used.

Techniques: Adsorption